The roster reflects the latest call up, announced by manager Fernando Santos on November 5, 2020.
Photo Cred: FPF
| Number | Player | Position | Club |
| 1 | Rui Patrício | Goalkeeper | Wolverhampton |
| 2 | Nélson Semedo | Defender | Wolverhampton |
| 3 | Rúben Semedo | Defender | Olympiacos |
| 4 | Rúben Dias | Defender | Manchester City |
| 5 | Raphaël Guerreiro | Defender | Borussia Dortmund |
| 6 | José Fonte | Defender | Lille |
| 7 | Cristiano Ronaldo (C) | Forward | Juventus |
| 8 | João Moutinho | Midfielder | Wolverhampton |
| 9 | Paulinho | Forward | Braga |
| 10 | Bernardo Silva | Forward | Manchester City |
| 11 | Bruno Fernandes | Midfielder | Manchester United |
| 12 | Anthony Lopes | Goalkeeper | Lyon |
| 13 | Danilo Pereira | Midfielder | Paris Saint-Germain |
| 14 | William Carvalho | Midfielder | Real Betis |
| 15 | Sérgio Oliveira | Midfielder | FC Porto |
| 16 | Pedro Neto | Forward | Wolverhampton |
| 17 | Francisco Trincão | Forward | Barcelona |
| 18 | Rúben Neves | Midfielder | Wolverhampton |
| 19 | Mário Rui | Defender | Napoli |
| 20 | João Cancelo | Defender | Manchester City |
| 21 | Diogo Jota | Forward | Liverpool |
| 22 | Rui Silva | Goalkeeper | Granada |
| 23 | João Félix | Forward | Atlético Madrid |
| - | Renato Sanches | Midfielder | Lille |
| - | Domingos Duarte | Defender | Granada |