This roster reflects the latest call up for EURO 2020, announced by manager Fernando Santos on May 20, 2021.
Photo Cred: FPF
| Player | Position | Club |
| Rui Patrício | Goalkeeper | Wolverhampton |
| Anthony Lopes | Goalkeeper | Lyon |
| Rui Silva | Goalkeeper | Granada |
| Rúben Dias | Defender | Manchester City |
| Pepe | Defender | FC Porto |
| José Fonte | Defender | Lille |
| Nélson Semedo | Defender | Wolverhampton |
| Raphaël Guerreiro | Defender | Borussia Dortmund |
| Nuno Mendes | Defender | Sporting CP |
| Diogo Dalot | Defender | Manchester United |
| Danilo Pereira | Midfielder | Paris Saint-Germain |
| Rúben Neves | Midfielder | Wolverhampton |
| João Palhinha | Midfielder | Sporting CP |
| William Carvalho | Midfielder | Real Betis |
| Bruno Fernandes | Midfielder | Manchester United |
| João Moutinho | Midfielder | Wolverhampton |
| Renato Sanches | Midfielder | Lille |
| Sérgio Oliveira | Midfielder | FC Porto |
| Pedro Gonçalves | Midfielder | Sporting CP |
| Cristiano Ronaldo (C) | Forward | Juventus |
| Bernardo Silva | Forward | Manchester City |
| Diogo Jota | Forward | Liverpool |
| João Félix | Forward | Atlético Madrid |
| André Silva | Forward | Eintracht Frankfurt |
| Rafa Silva | Forward | Benfica |
| Gonçalo Guedes | Forward | Valencia |
| *João Cancelo | Defender | Manchester City |
* Originally called up; removed from squad on June 13 due to COVID-19. Replaced by Diogo Dalot.